|
type |
Journal Article |
authors |
Manukhov, IV, Mamaeva, DV, Rastorguev, SM, Faleev, NG, Morozova, EA, Demidkina, TV, Zavilgelsky, GB. |
title |
A gene encoding L-methionine gamma-lyase is present in Enterobacteriaceae family genomes: identification and characterization of Citrobacter freundii L-methionine gamma-lyase |
journal |
J Bacteriol |
Activity |
4.4.1.11 |
Family |
4.4.1.11 |
sel |
selected |
ui |
15901718 |
year |
(2005) |
volume |
187 |
number |
11 |
pages |
3889-93 |
| |
abstract |
Citrobacter freundii cells produce L-methionine gamma-lyase when grown on a medium containing L-methionine. The nucleotide sequence of the hybrid plasmid with a C. freundii EcoRI insert of about 3.0 kbp contained two open reading frames, consisting of 1,194 nucleotides and 1,296 nucleotides, respectively. The first one (denoted megL) encoded L-methionine gamma-lyase. The enzyme was overexpressed in Escherichia coli and purified. The second frame encoded a protein belonging to the family of permeases. Regions of high sequence identity with the 3'-terminal part of the C. freundii megL gene located in the same regions of Salmonella enterica serovar Typhimurium, Shigella flexneri, E. coli, and Citrobacter rodentium genomes were found. |
last changed |
2009/07/01 14:09 |
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