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B6db references: 17979829

type Journal Article
authors Tang DW, Li LF, Yu YM, Liu XY, Su XD, Zhao X, Liang YH.
title Preparation, crystallization and preliminary X-ray analysis of threonine synthase from Streptococcus mutans
journal Protein Pept Lett
sel selected
ui 17979829
year (2007)
volume 14
number 8
pages 836-8
abstract The thrC gene of Streptococcus mutans encodes threonine synthase, which is a potential target for drug design. To study the structure and function of the enzyme, the thrC gene was amplified from Streptococcus mutans genomic DNA and cloned into the expression vector pET28alpha. The protein was expressed in Escherichia coli in soluble form and purified to homogeneity. Crystals suitable for X-ray diffraction were obtained by hanging-drop vapor diffusion method. The crystal diffracted to 2.5 A and belonged to space group P3(1) or P3(2), with unit cell parameters a=b=60.39 A, c=118.62 A.
last changed 2008/03/11 16:42

B6db references