|
type |
Journal Article |
authors |
Nozaki H, Kuroda S, Watanabe K, Yokozeki K. |
title |
Cloning of the gene encoding alpha-methylserine hydroxymethyltransferase from Aminobacter sp. AJ110403 and Ensifer sp. AJ110404 and characterization of the recombinant enzyme |
journal |
Biosci Biotechnol Biochem |
Activity |
2.1.2.7 |
Family |
2.1.2.7.a |
sel |
selected |
ui |
18997407 |
year |
(2008) |
volume |
72 |
number |
11 |
pages |
3002-5 |
| |
abstract |
Genes encoding alpha-methylserine hydroxymethyltransferase from Aminobacter sp. AJ110403 and Ensifer sp. AJ110404 were cloned and expressed in Escherichia coli. The purified enzymes were homodimers with a 46-kDa subunit and contained 1 mol/mol-subunit of pyridoxal 5'-phosphate. The V(max) of these enzymes catalyzing the conversion of alpha-methyl-L-serine to D-alanine via tetrahydrofolate was 22.1 U/mg (AJ110403) and 15.4 U/mg (AJ110404). |
last changed |
2018/11/22 11:46 |
|