Activities | Families | Sequences | Fold types | References | Help
B6db references: 18997407

type Journal Article
authors Nozaki H, Kuroda S, Watanabe K, Yokozeki K.
title Cloning of the gene encoding alpha-methylserine hydroxymethyltransferase from Aminobacter sp. AJ110403 and Ensifer sp. AJ110404 and characterization of the recombinant enzyme
journal Biosci Biotechnol Biochem
Activity 2.1.2.7
Family 2.1.2.7
sel selected
ui 18997407
year (2008)
volume 72
number 11
pages 3002-5
 
abstract Genes encoding alpha-methylserine hydroxymethyltransferase from Aminobacter sp. AJ110403 and Ensifer sp. AJ110404 were cloned and expressed in Escherichia coli. The purified enzymes were homodimers with a 46-kDa subunit and contained 1 mol/mol-subunit of pyridoxal 5'-phosphate. The V(max) of these enzymes catalyzing the conversion of alpha-methyl-L-serine to D-alanine via tetrahydrofolate was 22.1 U/mg (AJ110403) and 15.4 U/mg (AJ110404).
last changed 2009/02/26 19:08

B6db references