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B6db references: 19219581

type Journal Article
authors Jnawali HN, Subba B, Liou K, Sohng JK.
title Functional characterization of kanB by complementing in engineered Streptomyces fradiae Deltaneo6::tsr
journal Biotechnol Lett
sel selected
ui 19219581
year (2009)
volume 31
pages 869-75
abstract A putative aminotransferase gene, kanB, lies in the biosynthetic gene cluster of Streptomyces kanamyceticus ATCC 12853 and has 66% identity with neo6 in neomycin biosynthesis. Streptomyces fradiae Deltaneo6::tsr was generated by disrupting neo6 in the neomycin producer Streptomyces fradiae. Neomycin production was completely abolished in the disruptant mutant but was restored through self-complementation of neo6. S. fradiae HN4 was generated through complementation with kanB in Streptomyces fradiae neo6::tsr. Based on metabolite analysis by ESI/MS and LC/MS, neomycin production was restored in Streptomyces fradiae HN4. Thus, like neo6, kanB also functions as a 2-deoxy-scyllo-inosose aminotransferase that has dual functions in the formation of 2-deoxy-scyllo-inosose (DOS).
last changed 2017/11/03 17:51

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