|
type |
Journal Article |
authors |
Jnawali HN, Subba B, Liou K, Sohng JK. |
title |
Functional characterization of kanB by complementing in engineered Streptomyces fradiae Deltaneo6::tsr |
journal |
Biotechnol Lett |
Activity |
2.6.1.100 |
Family |
2.6.1.100 |
sel |
selected |
ui |
19219581 |
year |
(2009) |
volume |
31 |
pages |
869-75 |
| |
abstract |
A putative aminotransferase gene, kanB, lies in the biosynthetic gene cluster of Streptomyces kanamyceticus ATCC 12853 and has 66% identity with neo6 in neomycin biosynthesis. Streptomyces fradiae Deltaneo6::tsr was generated by disrupting neo6 in the neomycin producer Streptomyces fradiae. Neomycin production was completely abolished in the disruptant mutant but was restored through self-complementation of neo6. S. fradiae HN4 was generated through complementation with kanB in Streptomyces fradiae neo6::tsr. Based on metabolite analysis by ESI/MS and LC/MS, neomycin production was restored in Streptomyces fradiae HN4. Thus, like neo6, kanB also functions as a 2-deoxy-scyllo-inosose aminotransferase that has dual functions in the formation of 2-deoxy-scyllo-inosose (DOS). |
last changed |
2017/11/03 17:51 |
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