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B6db references: 21432644

type Journal Article
authors Wang, C. L.; Lum, A. M.; Ozuna, S. C.; Clark, D. S.; Keasling, J. D.
title Aerobic sulfide production and cadmium precipitation by Escherichia coli expressing the Treponema denticola cysteine desulfhydrase gene
journal Appl Microbiol Biotechnol
Activity Cysteine.desulfhydrase
ui 21432644
year (2001)
volume 56
number 3-4
pages 425-30.
 
keywords Aerobiosis
abstract The cysteine desulfhydrase gene of Treponema denticola was over- expressed in Escherichia coli to produce sulfide under aerobic conditions and to precipitate metal sulfide complexes on the cell wall. When grown in a defined salts medium supplemented with cadmium and cysteine, E. coli producing cysteine desulfhydrase secreted sulfide and removed nearly all of the cadmium from solution after 48 h. A control strain produced significantly less sulfide and removed significantly less cadmium. Measurement of acid-labile sulfide and energy dispersive X-ray spectroscopy indicated that cadmium was precipitated as cadmium sulfide. Without supplemental cysteine, both the E. coli producing cysteine desulfhydrase and the control E. coli demonstrated minimal cadmium removal.
last changed 2002/11/12 16:17

B6db references