|
type |
Journal Article |
authors |
Wang, C. L.; Lum, A. M.; Ozuna, S. C.; Clark, D. S.; Keasling, J. D. |
title |
Aerobic sulfide production and cadmium precipitation by Escherichia coli expressing the Treponema denticola cysteine desulfhydrase gene |
journal |
Appl Microbiol Biotechnol |
Activity |
4.4.1.28 |
ui |
21432644 |
year |
(2001) |
volume |
56 |
number |
3-4 |
pages |
425-30. |
| |
keywords |
Aerobiosis |
abstract |
The cysteine desulfhydrase gene of Treponema denticola was over- expressed in Escherichia coli to produce sulfide under aerobic conditions and to precipitate metal sulfide complexes on the cell wall. When grown in a defined salts medium supplemented with cadmium and cysteine, E. coli producing cysteine desulfhydrase secreted sulfide and removed nearly all of the cadmium from solution after 48 h. A control strain produced significantly less sulfide and removed significantly less cadmium. Measurement of acid-labile sulfide and energy dispersive X-ray spectroscopy indicated that cadmium was precipitated as cadmium sulfide. Without supplemental cysteine, both the E. coli producing cysteine desulfhydrase and the control E. coli demonstrated minimal cadmium removal. |
last changed |
2017/09/08 10:10 |
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