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B6db references: 21439480

type Journal Article
authors Wackler B, Schneider P, Jacobs JM, Pauly J, Allen C, Nett M, Hoffmeister D
title Ralfuranone biosynthesis in Ralstonia solanacearum suggests functional divergence in the quinone synthetase family of enzymes
journal Chem Biol
sel selected
ui 21439480
year (2011)
volume 18
number 3
pages 354-60
keywords doi: 10.1016/j.chembiol.2011.01.010.
abstract Ralstonia solanacearum is a destructive crop plant pathogen and produces ralfuranone, i.e., a monophenyl-substituted furanone. Extensive feeding experiments with (13)C-labeled L-phenylalanine now proved that all carbon atoms of the heterocycle derive, after deamination, from this aromatic amino acid. A genetic locus was identified which encodes the aminotransferase RalD and the furanone synthetase RalA. The latter is a tridomain nonribosomal peptide synthetase (NRPS)-like enzyme which was characterized (1) biochemically by the ATP-pyrophosphate exchange assay, and (2) genetically through gene inactivation and transcriptional analysis in axenic culture and in planta. This is the first study to our knowledge on the biochemical and genetic basis of R. solanacearum secondary metabolism. It implies new chemistry for NRPSs, as RalA-mediated biosynthesis requires C-C-bond and subsequent C-O-bond formation to establish the furanone ring system.
last changed 2019/05/14 11:41

B6db references