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B6db references: 24911465

type Journal Article
authors Singh S, Peltier-Pain P, Tonelli M, Thorson JS

title A general NMR-based strategy for the in situ characterization of sugar-nucleotide-dependent biosynthetic pathways
journal Org Lett
sel selected
ui 24911465
year (2014)
volume 16
number 12
pages 3220-3
keywords DOI: 10.1021/ol501241a
abstract A simple method for the study of sugar-nucleotide-dependent multienzyme cascades is highlighted where the use of selectively (13)C-labeled sugar nucleotides and inverse (13)C detection NMR offers fast, direct detection and quantification of reactants and products and circumvents the need for chromatographic separation. The utility of the method has been demonstrated by characterizing four previously uncharacterized sugar nucleotide biosynthetic enzymes involved in calicheamicin biosynthesis.
last changed 2019/06/18 13:26

B6db references