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B6db references: 25415663

type Journal Article
authors Shin SM, Kim H, Joo Y, Lee SJ, Lee YJ, Lee SJ, Lee DW
title Characterization of glutamate decarboxylase from Lactobacillus plantarum and its C-terminal function for the pH dependence of activity
journal J Agric Food Chem
Activity 4.1.1.15
Family 4.1.1.15.b
sel selected
ui 25415663
year (2014)
volume 62
number 50
pages 12186-93
 
keywords C-terminal region; GABA; Lactobacillus plantarum; glutamate decarboxylase; pH dependence
abstract The gadB gene encoding glutamate decarboxylase (GAD) from Lactobacillus plantarum was cloned and expressed in Escherichia coli. The recombinant enzyme exhibited maximal activity at 40 C and pH 5.0. The 3D model structure of L. plantarum GAD proposed that its C-terminal region (Ile454-Thr468) may play an important role in the pH dependence of catalysis. Accordingly, C-terminally truncated (Δ3 and Δ11 residues) mutants were generated and their enzyme activities compared with that of the wild-type enzyme at different pH values. Unlike the wild-type GAD, the mutants showed pronounced catalytic activity in a broad pH range of 4.0-8.0, suggesting that the C-terminal region is involved in the pH dependence of GAD activity. Therefore, this study may provide effective target regions for engineering pH dependence of GAD activity, thereby meeting industrial demands for the production of γ-aminobutyrate in a broad range of pH value.
last changed 2017/10/11 10:21

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