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B6db references: 25945710

type Journal Article
authors Abendroth J, Choi R, Wall A, Clifton MC, Lukacs CM, Staker BL, Van Voorhis W, Myler P, Lorimer DD, Edwards TE
title Structures of aspartate aminotransferases from Trypanosoma brucei, Leishmania major and Giardia lamblia
journal Acta Crystallogr F Struct Biol Commun
sel selected
ui 25945710
year (2015)
volume 71
number 5
pages 566-71
keywords Giardia lamblia; Leishmania major; Seattle Structural Genomics Center for Infectious Disease; Trypanosoma brucei; aspartate aminotransferase; pyridoxalphosphate lysine; structural genomics; transferase
abstract The structures of three aspartate aminotransferases (AATs) from eukaryotic pathogens were solved within the Seattle Structural Genomics Center for Infectious Disease (SSGCID). Both the open and closed conformations of AAT were observed. Pyridoxal phosphate was bound to the active site via a Schiff base to a conserved lysine. An active-site mutant showed that Trypanosoma brucei AAT still binds pyridoxal phosphate even in the absence of the tethering lysine. The structures highlight the challenges for the structure-based design of inhibitors targeting the active site, while showing options for inhibitor design targeting the N-terminal arm.
last changed 2017/09/14 09:04

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