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B6db references: 2674117

type Journal Article
authors Ogawa, H.; Gomi, T.; Konishi, K.; Date, T.; Nakashima, H.; Nose, K.; Matsuda, Y.; Peraino, C.; Pitot, H.C.; Fujioka, M.
title Human liver serine dehydratase. cDNA cloning and sequence homology with hydroxyamino acid dehydratases from other sources
journal J Biol Chem
Activity 4.3.1.17
Family 4.3.1.17a
sel selected
ui 2674117
year (1989)
volume 264
number 27
pages 15818-23
 
abstract Rat liver serine dehydratase cDNA was used to screen a human liver cDNA library in lambda gt11. One positive clone occurred in every 5,000 clones. Fifteen positive clones were plaque purified. The largest cDNA obtained contained an open reading frame of 987 base pairs, and 5' and 3' noncoding regions of 89 and 317 base pairs, respectively. The deduced amino acid sequence, with a calculated Mr of 34,615, was similar to that of rat liver serine dehydratase except for the absence of a segment consisting of 36 amino acid residues. In vitro transcription/translation with the cDNA resulted in the formation of a polypeptide with an Mr of approximately 35,000, which cross-reacted with the anti-rat serine dehydratase antibody. These results suggest that the human serine dehydratase is structurally cognate with the rat enzyme. Moreover, portions of the sequence postulated to be essential for activity in microbial threonine dehydratases are found in the mammalian serine dehydratases, suggesting that hydroxyamino and dehydratases may have originated from a common ancestor.
last changed 2014/03/05 15:16

B6db references