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B6db references: 2832253

type Journal Article
authors Park, Y. M.; Stauffer, G. V.
title Cloning and characterization of the metC gene from Salmonella typhimurium LT2
journal Gene
sel selected
ui 2832253
year (1987)
volume 60
number 2-3
pages 291-7
keywords Chromosome Mapping
abstract The metC gene of Salmonella typhimurium was cloned into the plasmid vectors pACYC184 and pBR322. Genetic and biochemical experiments indicate that the region controlling metC gene expression is present on the cloned fragments. The location of the metC gene was determined by insertional inactivation with transposons Tn5 and mini-Mu. The gene product was identified in a minicell system as a 49-kDa polypeptide. The direction of transcription and translation was determined by correlating the orientation of mini-Mu insertions within the metC gene with the expression of the lacZ gene contained in mini-Mu.
last changed 2009/07/01 13:05

B6db references