|
type |
Journal Article |
authors |
Beasley JT, Bonneau JP, Johnson AAT |
title |
Characterisation of the nicotianamine aminotransferase and deoxymugineic acid
synthase genes essential to Strategy II iron uptake in bread wheat (Triticum
aestivum L.)
|
journal |
PLoS One |
Activity |
2.6.1.80 |
Family |
2.6.1.80 |
sel |
selected |
ui |
28475636 |
year |
(2017) |
volume |
12 |
number |
5 |
pages |
e0177061 |
| |
keywords |
doi: 10.1371/journal.pone.0177061 |
abstract |
Iron (Fe) uptake in graminaceous plant species occurs via the release and
uptake of Fe-chelating compounds known as mugineic acid family
phytosiderophores (MAs). In the MAs biosynthetic pathway, nicotianamine
aminotransferase (NAAT) and deoxymugineic acid synthase (DMAS) enzymes catalyse
the formation of 2'-deoxymugineic acid (DMA) from nicotianamine (NA). Here we
describe the identification and characterisation of six TaNAAT and three
TaDMAS1 genes in bread wheat (Triticum aestivum L.). The coding sequences of
all six TaNAAT homeologs consist of seven exons with .0% nucleotide sequence
identity and most sequence variation present in the first exon. The coding
sequences of the three TaDMAS1 homeologs consist of three exons with .8%
nucleotide sequence identity. Phylogenetic analysis revealed that the TaNAAT
and TaDMAS1 proteins are most closely related to the HvNAAT and HvDMAS1
proteins of barley and that there are two distinct groups of TaNAAT proteins-
TaNAAT1 and TaNAAT2 -that correspond to the HvNAATA and HvNAATB proteins,
respectively. Quantitative reverse transcription-PCR analysis revealed that the
TaNAAT2 genes are expressed at highest levels in anther tissues whilst the
TaNAAT1 and TaDMAS1 genes are expressed at highest levels in root tissues of
bread wheat. Furthermore, the TaNAAT1, TaNAAT2 and TaDMAS1 genes were
differentially regulated by plant Fe status and their expression was
significantly upregulated in root tissues from day five onwards during a seven-
day Fe deficiency treatment. The identification and characterization of the
TaNAAT1, TaNAAT2 and TaDMAS1 genes provides a valuable genetic resource for
improving bread wheat growth on Fe deficient soils and enhancing grain Fe
nutrition.
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last changed |
2017/11/17 13:17 |
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