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B6db references: 29024617.1

type Journal Article
authors Phillips RS, Poteh P, Miller KA, Hoover TR
title STM2360 encodes a d-ornithine/d-lysine decarboxylase in Salmonella enterica serovar typhimurium
journal Arch Biochem Biophys
sel selected
ui 29024617.1
year (2017)
volume 634
pages 83-87
keywords Decarboxylase; Pyridoxal-5′-phosphate; Racemase; d-amino acid
abstract STM2360 is a gene located in a small operon of undetermined function in Salmonella enterica serovar Typhimurium LT2. The amino acid sequence of STM2360 shows significant similarity (∼30% identity) to diaminopimelate decarboxylase (DapDC), a Fold III pyridoxal-5'-phosphate (PLP) dependent enzyme involved in l-lysine biosynthesis. We have found that the protein coded by STM2360 has a previously undocumented catalytic activity, d-ornithine/d-lysine decarboxylase (DOKDC). The reaction products, cadaverine and putrescine, respectively, were identified by NMR and mass spectrometry. The substrate specificity of DOKDC is d-Lysine > d-Ornithine. This is the first pyridoxal-5'-phosphate dependent decarboxylase identified to act on d-amino acids. STM2358, located in the same operon, has ornithine racemase activity. This suggests that the physiological substrate of the decarboxylase and the operon is ornithine. Homologs of STM2360 with high sequence identity (>80%) are found in other common enterobacteria, including species of Klebsiella, Citrobacter, Vibrio and Hafnia, as well as Clostridium in the Firmicutes, and Pseudomonas.
last changed 2017/12/15 11:56

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