|
type |
Journal Article |
authors |
Brown HA, Vinogradov E, Gilbert M, Holden HM |
title |
The Mycobacterium tuberculosis Complex has a Pathway for the
Biosynthesis of 4-Formamido-4,6-Dideoxy-D-Glucose |
journal |
Protein Science |
Activity |
2.6.1.33 |
Family |
2.6.1.33.b |
sel |
selected |
ui |
29761597 |
year |
(2018) |
volume |
27 |
number |
8 |
pages |
1491-1497 |
| |
keywords |
aminotransferase, dTDP-4-amino-4,6-dideoxy-D-glucose, Mycobacterium tuberculosis, pyridoxal 5'-phosphate, Rv3402c. |
abstract |
Recent studies have demonstrated that the O-antigens of some pathogenic
bacteria such as Brucella abortus, Francisella tularensis, and Campylobacter
jejuni contain quite unusual N-formylated sugars (3-formamido-3,6-dideoxy-d-
glucose or 4-formamido-4,6-dideoxy-d-glucose). Typically, four enzymes are
required for the formation of such sugars: a thymidylyltransferase, a 4,6-
dehydratase, a pyridoxal 5'-phosphate or PLP-dependent aminotransferase, and
an N-formyltransferase. To date, there have been no published reports of N-
formylated sugars associated with Mycobacterium tuberculosis. A recent
investigation from our laboratories, however, has demonstrated that one gene
product from M. tuberculosis, Rv3404c, functions as a sugar N-
formyltransferase. Given that M. tuberculosis produces l-rhamnose, both a
thymidylyltransferase (Rv0334) and a 4,6-dehydratase (Rv3464) required for
its formation have been identified. Thus, there is one remaining enzyme
needed for the production of an N-formylated sugar in M. tuberculosis,
namely a PLP-dependent aminotransferase. Here we demonstrate that the M.
tuberculosis rv3402c gene encodes such an enzyme. Our data prove that M.
tuberculosis contains all of the enzymatic activities required for the
formation of dTDP-4-formamido-4,6-dideoxy-d-glucose. Indeed, the rv3402c
gene product likely contributes to virulence or persistence during
infection, though its temporal expression and location remain to be
determined. |
fulltext |
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last changed |
2019/06/03 09:49 |
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