|
type |
Journal Article |
authors |
Wang, M. D.; Liu, L.; Wang, B. M.; Berg, C. M. |
title |
Cloning and characterization of the Escherichia coli K-12 alanine- valine transaminase (avtA) gene |
journal |
J Bacteriol |
Activity |
2.6.1.66 |
Family |
2.6.1.66.a |
sel |
selected |
ui |
3040683 |
year |
(1987) |
volume |
169 |
number |
9 |
pages |
4228-34 |
| |
keywords |
Chromosome Mapping |
abstract |
avtA, which encodes the alanine-valine transaminase, transaminase C, was cloned in vivo with high- and low-copy-number mini-Mu cloning vectors. The phenotype conferred by the cloned avtA+ gene usually depended upon the plasmid copy number; most high-copy-number avtA+ plasmids permitted isoleucine-requiring ilvE strains to grow in the absence of isoleucine (multicopy suppression), while low-copy-number avtA+ plasmids did not. avtA was mapped to a 1.25-kilobase segment by comparison of the restriction maps of 24 independent mini-Mu plasmids and then by gamma-delta (Tn1000) mutagenesis of a pBR322-avtA+ plasmid. The direction of transcription of avtA on the cloned fragment was determined with fusions to a promoterless lac gene. |
last changed |
2019/06/19 15:37 |
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