|
type |
Journal Article |
authors |
Dai GZ, Han WB, Mei YN, Xu K, Jiao RH, Ge HM, Tan RX |
title |
Pyridoxal-5'-phosphate-dependent bifunctional enzyme catalyzed biosynthesis of indolizidine alkaloids in fungi |
journal |
Proc Natl Acad Sci U S A. |
Activity |
cuab |
Family |
cuab |
sel |
selected |
ui |
31882449 |
year |
(2020) |
volume |
117 |
number |
2 |
pages |
1174-1180 |
| |
keywords |
doi: 10.1073/pnas.1914777117 |
abstract |
Indolizidine alkaloids are widespread in nature, diverse in structure, prominent in bioactivity, and elusive in ecology, but only a few have been biosynthetically clarified. Here, we describe the biosynthesis of curvulamine, a unique antibacterial indolizidine alkaloid from Curvularia sp. IFB-Z10. Unexpectedly, curvulamine results from a consortium of 6 enzymes including a pyridoxal-5′-phosphate (PLP)-dependent bifunctional aminotransferase (CuaB) catalyzing the formation of C−C (via Claisen condensation) and C−O bonds (using dioxygen). Guided by the CuaB function, our genome mining effort revealed, from a single silent gene cluster, a collection of previously undescribed polyketide alkaloids with 1 more antibacterial than curvulamine. Collectively, the work describes an unprecedented construction of indolizidine-coined skeletons and provides another layer of the functional versatility to the PLP-dependent enzymes. |
fulltext |
file.pdf (1,302,033 bytes) |
last changed |
2020/02/17 09:40 |
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