|
type |
Journal Article |
authors |
Meadows, G. G.; DiGiovanni, J.; Minor, L.; Elmer, G. W. |
title |
Some biological properties and an in vivo evaluation of tyrosine phenol- lyase on growth of B-16 melanoma |
journal |
Cancer Res |
Activity |
4.1.99.2 |
ui |
76116118 |
year |
(1976) |
volume |
36 |
number |
1 |
pages |
167-71. |
| |
keywords |
Animal |
abstract |
Tyrosine phenol-lyase from Erwinia herbicola was purified with the goal of assessing its effect on growth of malignant melanoma. Ammonium sulfate-sodium citrate fractionation and diethylaminoethyl cellulose- hydroxylapatite chromatography were used. The purified enzyme was shown to reduce plasma tyrosine levels when administered to normal C57BL x DBA/2 F1 mice. The plasma half-life value of the enzyme was found to be 6 to 7 hr. Unlike results reported with glutaminase and asparaginase preparations, the lactate dehydrogenase-elevating virus had no significant influence on plasma clearance of tyrosine phenol-lyase. The enzyme significantly inhibited growth of established B-16 melanoma tumors. |
last changed |
2002/11/12 16:17 |
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