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B6db references: 76202620

type Journal Article
authors Galas, E.; Florianowicz, T.
title L-Glutamate-glyoxylate aminotransferase in Lactobacillus plantarum
journal Acta Microbiol Pol B
ui 76202620
year (1975)
volume 7
number 4
pages 243-52
keywords Azides/pharmacology
abstract Glutamate-glyoxylate aminotransferase which mediates the reaction of glyoxylic acid with glutamic acid to yield glycine and alpha- oxoglutaric acid has been isolated and purified 84-fold from extracts of Lactobacillus plantarum. Purified enzyme requires the addition of pyridoxal phosphate and magnesium ions for its activity. The molecular weight of the enzyme estimated by Sepharose 4B gel filtration amounts to 37.000. Micaelis constants for glyoxylate and glutamate are corresponding to 6.25 X 10(-3) M and 2.75 X 10(-3) M, respectively. Optimal pH in phosphate and veronal buffers is 8.0 and optimal temperature 35--37 degrees C.
last changed 2002/11/12 16:17

B6db references