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B6db references: 77158018

type Journal Article
authors Yamaguchi, T.; Matsumura, Y.
title Purification of L-glutamate decarboxylase by affinity chromatography
journal Biochim Biophys Acta
ui 77158018
year (1977)
volume 481
number 2
pages 706-11.
keywords Animal
abstract L-Glutamate decarboxylase (L-glutamate 1-carboxy-lyase, EC from rat brain synaptosomal extract was partially purified by affinity chromatography. On further purification by DEAE-Sephadex A 50 and Sephadex G-200, L-glutamate decarboxylase was purified to greater extent. It was found that a single affinity chromatography by appropriate elution gave a highly purified protein giving a single band of high specific activity on polyacrylamide gradient gel slab electrophoresis with minimal contamination. Substrate specificity of the purified enzyme was modified in the presence of 6-azauracil or phenylalanine resulting in decreased specificity to L-glutamate and increased specificity to L-aspartate.
last changed 2002/11/12 16:17

B6db references