|
type |
Journal Article |
authors |
Hodsdon, J.; Kolb, H.; Snell, E. E.; Cole, R. D. |
title |
The pyridoxal-binding site in pyridoxamine-pyruvate transaminase |
journal |
Biochem J |
Activity |
2.6.1.30 |
ui |
78123679 |
year |
(1978) |
volume |
169 |
number |
2 |
pages |
429-32. |
| |
keywords |
Amino Acids/analysis |
abstract |
The enzyme-substrate complex formed between pyridoxamine-pyruvate transaminase (EC 2.6.1.30) and pyridoxal was reduced with NaBH4. After carboxymethylation and tryptic digestion, pyridoxyl-lysine-containing peptides were isolated by a combination of Sephadex and Dowex 50 chromatography. Analysis of these peptides shows the structure around the pyridoxal-binding lysine residues to be Ala-Asp-Ile-Tyr-Val-Thr-Gly- Pro-Asx-Lys(Pxy)-Cys-Leu(Pro2, Gly2, Ala2, Met)(Thr, Leu2)Gly-Val-Ser- Glu-Arg. This structure differs from those found for the corresponding peptides from pyridoxal phosphate-dependent enzymes. |
last changed |
2002/11/13 16:05 |
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