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B6db references: 79088693

type Journal Article
authors Kikuchi, S.; Ishimoto, M.
title A D-serine dehydratase acting also on L-serine from Klebsiella pneumoniae
journal J Biochem (Tokyo)
ui 79088693
year (1978)
volume 84
number 5
pages 1133-8.
keywords Enzyme Induction
abstract D-Serine dehydratase [EC] was purified from a strain of Klebsiella pneumoniae 140-fold from crude extract with a yield of 5%. This enzyme catalyzed formation of pyruvate and ammonia not only from D- serine but also from L-serine, and also catalyzed the formation of alpha-ketobutyrate and ammonia from D-threonine. Km values for D- serine, L-serine, and D-threonine were 2.8 mM, 20 mM, and 3.6 mM, respectively. Km for pyridoxal 5'-phosphate was 2.5 micron. The molecular weight was estimated to be 46,000 by Sephadex G-150 gel filtration and 40,000 by SDS-polyacrylamide gel electrophoresis. This enzyme was inducible by D-serine. Induction by casamino acids appeared to depend on the presence of D-serine.
last changed 2002/11/18 16:09

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