|
type |
Journal Article |
authors |
Kikuchi, S.; Ishimoto, M. |
title |
A D-serine dehydratase acting also on L-serine from Klebsiella pneumoniae |
journal |
J Biochem (Tokyo) |
Activity |
4.3.1.18 |
ui |
79088693 |
year |
(1978) |
volume |
84 |
number |
5 |
pages |
1133-8. |
| |
keywords |
Enzyme Induction |
abstract |
D-Serine dehydratase [EC 4.2.1.14] was purified from a strain of Klebsiella pneumoniae 140-fold from crude extract with a yield of 5%. This enzyme catalyzed formation of pyruvate and ammonia not only from D- serine but also from L-serine, and also catalyzed the formation of alpha-ketobutyrate and ammonia from D-threonine. Km values for D- serine, L-serine, and D-threonine were 2.8 mM, 20 mM, and 3.6 mM, respectively. Km for pyridoxal 5'-phosphate was 2.5 micron. The molecular weight was estimated to be 46,000 by Sephadex G-150 gel filtration and 40,000 by SDS-polyacrylamide gel electrophoresis. This enzyme was inducible by D-serine. Induction by casamino acids appeared to depend on the presence of D-serine. |
last changed |
2002/11/18 16:09 |
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