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B6db references: 80265182

type Journal Article
authors Ramesh, K. S.; Appaji Rao, N.
title Inhibition of monkey liver serine hydroxymethyltransferase by Cibacron Blue 3G-A
journal Biochem J
ui 80265182
year (1980)
volume 187
number 1
pages 249-52.
keywords Animal
abstract Cibacron Blue 3G-A inhibited monkey liver serine hydroxymethyltransferase competitively with respect to tetrahydrofolate and non-competitively with respect to L-serine. NADH, a positive heterotropic effector, failed to protect the enzymes against inhibition by the dye and was unable to desorb the enzyme from Blue Sepharose CL- 6B gel matrix. The binding of the dye to the free enzyme was confirmed by changes in the dye absorption spectrum. The results indicate that the dye probably binds at the tetrahydrofolate-binding domain of the enzyme, rather than at the 'dinucleotide fold'.
last changed 2002/11/04 17:41

B6db references