|
type |
Journal Article |
authors |
Lam, V. M.; Chan, I. P.; Yeung, Y. G. |
title |
Role of L-threonine deaminase and L-threonine 3-dehydrogenase in the utilization of L-threonine by Pseudomonas aeruginosa |
journal |
J Gen Microbiol |
Activity |
4.3.1.19 |
Family |
4.3.1.19 |
ui |
81027443 |
year |
(1980) |
volume |
117 |
number |
2 |
pages |
539-42. |
| |
keywords |
Alcohol Oxidoreductases/*metabolism |
abstract |
Mutants of Pseudomonas aeruginosa PAC1 which could grow on L-threonine were isolated. These mutants, like the parent strain, synthesized a biosynthetic threonine deaminase, but its apparent Km value for threonine was higher than that of the enzyme from strain PAC1. These mutants also synthesized an inducible NAD-dependent threonine dehydrogenase, which was not present in the parent strain. No threonine aldolase activity could be detected. The results suggest that the threonine deaminase with lowered affinity for L-threonine, together with L-threonine dehydrogenase, enabled these mutants to utilize L- threonine as the sole source of carbon for growth. |
last changed |
2008/04/01 16:02 |
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