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B6db references: 83203977

type Journal Article
authors Bailey, C. J.; Turner, P. D.
title Purification and properties of tryptophan synthase from baker's yeast (Saccharomyces cerevisiae)
journal Biochem J
ui 83203977
year (1983)
volume 209
number 1
pages 151-7.
keywords Amino Acids/analysis
abstract Tryptophan synthase was purified from baker's yeast. The purified enzyme exhibited one band on polyacrylamide-gel electrophoresis, had no detectable N-terminal amino acid and C-terminal alanine. The amino acid composition was close to that predicted by recent studies on the DNA sequence of the structural gene for the enzyme. Kinetic parameters for the following three activities were measured: indole-serine condensation, indolylglycerol phosphate lyase and the overall reaction of serine with 1-(indol-3-yl)glycerol 3-phosphate. The Km for indole was much lower than suggested by previous investigations, and the value of 11 microM was measured by a fluorimetric assay.
last changed 2002/11/12 16:17

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