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B6db references: 83210198

type Journal Article
authors Iwamoto, R.; Imanaga, Y.; Sawada, S.; Soda, K.
title Stereochemistry of an alpha, beta-elimination reaction by D- glucosaminate dehydratase
journal FEBS Lett
ui 83210198
year (1983)
volume 156
number 1
pages 33-6.
keywords Agrobacterium/enzymology
abstract The proton NMR analysis of D-glucosaminate dehydratase reaction in D2O revealed the incorporation of a deuterium atom at C-3 carbon of the product, 2-keto-3-deoxy-D-gluconate. Based on the chemical shift of C-3 proton of the product and the coupling constant characteristic for the C-3 and C-4 axial-axial coupling in the 2C5 pyranose conformation, the deuterium is in the pro-S position. Thus, the dehydration of D- glucosaminate by the enzyme proceeds in a retention mode at C-3 carbon. Kinetic parameters show that the rate-determining step is the abstraction of alpha-proton from the substrate.
last changed 2004/04/16 21:18

B6db references