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B6db references: 84107549

type Journal Article
authors Watts, S. D.; Atkins, A. M.
title Application of a quick, simple and direct radiometric assay for 4- aminobutyrate:2-oxoglutarate aminotransferase to studies of the parasitic nematode Nippostrongylus brasiliensis
journal Comp Biochem Physiol B
ui 84107549
year (1983)
volume 76
number 4
pages 899-906
keywords 4-Aminobutyrate Transaminase/*analysis/antagonists & inhibitors/metabolism
abstract A preparation of rat brain was used to develop a quick and simple radiometric assay for 4-aminobutyrate: 2-oxoglutarate aminotransferase (GABA-T), an important enzyme in neural tissue of vertebrates and invertebrates. Application of the methodology to the parasitic nematode Nippostrongylus brasiliensis revealed that a soluble preparation of partially purified GABA-T could be recovered in high yield. This enzyme had a specific activity comparable to that observed in rat brain after similar treatment, was very stable when frozen, depended for activity upon tightly-bound pyridoxal 5-phosphate, had an apparent molecular weight of 72,000 and was strongly inhibited by NaCl. The inhibition was competitive with 4-aminobutyrate (Ki = 20 mM) but uncompetitive with 2- oxoglutarate (Ki = 160 mM).
last changed 2002/11/04 17:41

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