|
type |
Journal Article |
authors |
Watts, S. D.; Atkins, A. M. |
title |
Application of a quick, simple and direct radiometric assay for 4- aminobutyrate:2-oxoglutarate aminotransferase to studies of the parasitic nematode Nippostrongylus brasiliensis |
journal |
Comp Biochem Physiol B |
Activity |
2.6.1.19 |
ui |
84107549 |
year |
(1983) |
volume |
76 |
number |
4 |
pages |
899-906 |
| |
keywords |
4-Aminobutyrate Transaminase/*analysis/antagonists & inhibitors/metabolism |
abstract |
A preparation of rat brain was used to develop a quick and simple radiometric assay for 4-aminobutyrate: 2-oxoglutarate aminotransferase (GABA-T), an important enzyme in neural tissue of vertebrates and invertebrates. Application of the methodology to the parasitic nematode Nippostrongylus brasiliensis revealed that a soluble preparation of partially purified GABA-T could be recovered in high yield. This enzyme had a specific activity comparable to that observed in rat brain after similar treatment, was very stable when frozen, depended for activity upon tightly-bound pyridoxal 5-phosphate, had an apparent molecular weight of 72,000 and was strongly inhibited by NaCl. The inhibition was competitive with 4-aminobutyrate (Ki = 20 mM) but uncompetitive with 2- oxoglutarate (Ki = 160 mM). |
last changed |
2002/11/04 17:41 |
|