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B6db references: 84161899

type Journal Article
authors Barnett, G. R.; Kazarinoff, M. N.
title Purification and properties of ornithine decarboxylase from Physarum polycephalum
journal J Biol Chem
ui 84161899
year (1984)
volume 259
number 1
pages 179-83.
keywords Chromatography, High Pressure Liquid
abstract Ornithine decarboxylase (EC has been purified 3,500-fold from the plasmodia of Physarum polycephalum. The purified material exhibited a Km for ornithine of 0.6 mM and Vmax of 20 mumol of CO2 formed per min/mg at 30 degrees C (62 mumol/min/mg at 37 degrees C). It migrated as a single protein and activity species on high pressure liquid chromatography (TSK-3000) in 0.15 M NaCl (Mr = 80,000) and in native gels containing 5, 6.5, 8, and 9.5% acrylamide. A single protein band (Mr = 43,000) was observed on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Activity was lost upon incubation with alpha- difluoromethyl[5-14C] ornithine, and the inactivated material appeared as a single Mr = 43,000 14C-band on autoradiograms of sodium dodecyl sulfate-polyacrylamide gels. The decarboxylase activity was specific for ornithine and was pyridoxal-P-dependent. The Km for pyridoxal-P (10 microM) was identical with the Kd for pyridoxal-P binding determined from the quenching of protein fluorescence (lambda ex = 282 nm, lambda em = 350 nm, maximal quenching 81%). Using specific antibody obtained from rabbit hyperimmune serum as a probe, an Mr = 43,000 immunoreactive species was detected on nitrocellulose blots of sodium dodecyl sulfate- polyacrylamide gels of plasmodial homogenates and all pooled purification fractions, but no higher molecular weight cross-reactive material was detected.
last changed 2002/11/12 16:17

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