Activities | Families | Sequences | Fold types | References | Help
B6db references: 84182898

type Journal Article
authors Deshmukh, D. R.; Srivastava, S. K.
title Purification and properties of ornithine aminotransferase from rat brain
journal Experientia
ui 84182898
year (1984)
volume 40
number 4
pages 357-9.
keywords Amino Acids, Branched-Chain/pharmacology
abstract Ornithine aminotransferase (E.C. from rat brain was purified 100-fold by ammonium sulphate fractionation, DEAE cellulose chromatography, calcium phosphate gel and alumina C gamma gel. Pyridoxal phosphate was essential for maximum activity of the enzyme. The brain enzyme did not differ from liver and kidney enzymes in properties such as pH optimum, Km, substrate specificity and the inhibition by branched chain amino acids. Unlike rat liver enzyme, brain ornithine aminotransferase was able to catalyze the reaction between L-lysine and 2-oxoglutarate. Spermidine and spermine inhibited brain ornithine aminotransferase activity.
last changed 2002/11/04 17:41

B6db references