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B6db references: 84214945

type Journal Article
authors Boronat, A.; Britton, P.; Jones-Mortimer, M. C.; Kornberg, H. L.; Lee, L. G.; Murfitt, D.; Parra, F.
title Location on the Escherichia coli genome of a gene specifying O- acetylserine (thiol)-lyase
journal J Gen Microbiol
ui 84214945
year (1984)
volume 130
number Pt 3
pages 673-85.
keywords Amino Acids/analysis
abstract The plasmid pAB65, derived from a specialized transducing phage carrying DNA from about 52 min on the Escherichia coli genome, coded for two polypeptides of Mr approx. 34 000. The expression of one was regulated by cyst(e)ine and the cysB gene product and the other by the cysB gene product only. One of these polypeptides was a subunit of O- acetylserine (thiol)-lyase (EC; the other, associated with the E. coli membrane, was the N-terminus of the product of the lambda ben gene. The pattern of peptide synthesis directed by plasmids carrying smaller DNA fragments indicated that the gene for O- acetylserine (thiol)-lyase was transcribed clockwise. The spectrum, amino acid composition and subunit number of the enzyme were determined. The enzyme appears homologous with the Salmonella typhimurium cysK gene product. This provides further evidence for the inversion of this region of the genome.
last changed 2003/03/17 14:53

B6db references