|
type |
Journal Article |
authors |
Korpela, T. K.; Saarinen, R. |
title |
Affinity chromatography of B6-vitamin-dependent enzymes: purification of pig-heart branched-chain amino acid transaminase |
journal |
J Chromatogr |
Activity |
2.6.1.42 |
ui |
85157974 |
year |
(1985) |
volume |
318 |
number |
2 |
pages |
333-41. |
| |
keywords |
Animal |
abstract |
Pig-heart branched-chain amino acid transaminase (EC 2.6.1.42) was purified to near homogeneity with a yield of 27%. A prepurification was performed by heat treatment, gel chromatography and DEAE-Sepharose methods. For the final step, several affinity gels were tested and the one containing cycloserine coupled to CNBr-activated Sepharose 4B was selected. This effected an additional five-fold purification with a yield of 60%. The present affinity results are compared with corresponding studies with other aminotransferases in an attempt to find possible universal techniques for their purification. |
last changed |
2002/11/12 16:17 |
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