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B6db references: 85285615

type Journal Article
authors Shanker, J.; Datta, K.
title Evidence of hemin as an end product inhibitor of L-alanine: 4,5- dioxovalerate transaminase in rat liver mitochondria
journal FEBS Lett
ui 85285615
year (1985)
volume 189
number 1
pages 129-32.
keywords Alanine/metabolism
abstract This study describes the in vitro and in vivo effect of hemin on L- alanine:4,5-dioxovalerate transaminase activity. Hemin was shown to be an inhibitor of the purified enzyme and this inhibition was proportional to the concentration of hemin. The examined kinetic data with hemin showed uncompetitive inhibition for both alanine and 4,5- dioxovalerate. An apparent Ki of 30 and 42 microM for hemin were obtained with both alanine and 4,5-dioxovalerate, respectively. Moreover, the enzyme activity in liver was considerably decreased after the intravenous hemin administration and such an inhibition is dose and time dependent. Furthermore, maximum inhibition of the enzyme was observed 30 min after hemin injection and 60% enzyme inhibition was achieved with a dose of 1.2 mg/kg body wt of rat. Thus is suggests the important role of this enzyme on heme biosynthesis.
last changed 2002/11/12 16:17

B6db references