|
type |
Journal Article |
authors |
Kimura, H.; Izawa, M.; Sumino, Y. |
title |
Molecular analysis of the gene cluster involved in cephalosporin biosynthesis from Lysobacter lactamgenus YK90 |
journal |
Appl Microbiol Biotechnol |
Activity |
5.1.1.17 |
Family |
5.1.1.17 |
sel |
selected |
ui |
8703429 |
year |
(1996) |
volume |
44 |
number |
5 |
pages |
589-96 |
| |
keywords |
Sequence |
abstract |
Determination of the nucleotide sequence downstream from the Lysobacter lactamgenus pcbC gene encoding isopenicillin N synthase revealed that five open-reading frames (ORF) including the pcbC gene were tightly linked in the same orientation. Each ORF has the remarkable feature of the protein-coding frame in the DNA sequence with a high G+C content. Expression in Escherichia coli and a comparison of the deduced amino acid sequences with published sequences showed that the gene cluster contained a deacetoxycephalosporin C synthetase (DAOCS) gene (cefE), an ORF having homology with the Cephalosporium acremonium DAOCS/deacetylcephalosporin C synthetase gene (cefEF), an isopenicillin N epimerase gene(cefD), and a beta-lactamase gene. The gene order was pcbC-cefE-ORF3-cefD-beta-lactamase. |
last changed |
2009/07/03 14:29 |
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