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B6db references: 8703429

type Journal Article
authors Kimura, H.; Izawa, M.; Sumino, Y.
title Molecular analysis of the gene cluster involved in cephalosporin biosynthesis from Lysobacter lactamgenus YK90
journal Appl Microbiol Biotechnol
Activity 5.1.1.17
Family 5.1.1.17
sel selected
ui 8703429
year (1996)
volume 44
number 5
pages 589-96
 
keywords Sequence
abstract Determination of the nucleotide sequence downstream from the Lysobacter lactamgenus pcbC gene encoding isopenicillin N synthase revealed that five open-reading frames (ORF) including the pcbC gene were tightly linked in the same orientation. Each ORF has the remarkable feature of the protein-coding frame in the DNA sequence with a high G+C content. Expression in Escherichia coli and a comparison of the deduced amino acid sequences with published sequences showed that the gene cluster contained a deacetoxycephalosporin C synthetase (DAOCS) gene (cefE), an ORF having homology with the Cephalosporium acremonium DAOCS/deacetylcephalosporin C synthetase gene (cefEF), an isopenicillin N epimerase gene(cefD), and a beta-lactamase gene. The gene order was pcbC-cefE-ORF3-cefD-beta-lactamase.
last changed 2009/07/03 14:29

B6db references