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B6db references: 87250349

type Journal Article
authors Masuda, T.; Sakamoto, M.; Nishizaki, I.; Hayashi, H.; Yamamoto, M.; Wada, H.
title Affinity purification and characterization of serine hydroxymethyltransferases from rat liver
journal J Biochem (Tokyo)
ui 87250349
year (1987)
volume 101
number 3
pages 643-52.
keywords Animal
abstract A rapid and simple method was developed for the purification of serine hydroxymethyltransferases [EC]. The procedure involved ammonium sulfate precipitation, DEAE-cellulose column chromatography and affinity chromatography on an L-adsorbent. Through this procedure the cytosolic enzyme (s-SHMT) was purified 1,650-fold, and the mitochondrial enzyme (m-SHMT) 1,730-fold, with a yield of more than 30% in both cases. Both preparations gave a single band with a Mr of 54,000 on SDS-PAGE. The native enzymes both contained 4 mol of pyridoxal phosphate/mol of enzyme, and showed a Mr value of 220,000 on gel filtration, indicating a tetrameric structure. Several other properties of the enzymes were also studied.
last changed 2002/11/04 17:41

B6db references