type	Journal Article
authors	Fujiwara, K.; Okamura-Ikeda, K.; Motokawa, Y.
title	Amino acid sequence of the phosphopyridoxyl peptide from P-protein of the chicken liver glycine cleavage system
journal	Biochem Biophys Res Commun
Activity	2.1.2.1
ui	88106483
year	(1987)
volume	149
number	2
pages	621-7.
keywords	Amino Acid Oxidoreductases/*analysis
abstract	A pyridoxal 5'-phosphate-containing peptide which contained 54 amino acid residues was isolated from chicken liver P-protein of the glycine cleavage system following reduction with NaB3H4, carboxymethylation, and proteolysis with lysylendopeptidase. Two peptides which comprise the two halves of the phosphopyridoxyl peptide were isolated from apo-P- protein. Sequence analysis of these three peptides provided the primary structure of the phosphopyridoxyl peptide and revealed that the cofactor is linked to Lys-35. The pyridoxal 5'-phosphate-binding site has the His-Lys(PLP)-X structure characteristic of known pyridoxal 5'- phosphate-dependent amino acid decarboxylases, tryptophan synthase, and serine hydroxymethyltransferase.
last changed	2002/11/04 17:41