|
type |
Journal Article |
authors |
Gugler, K.; Funk, C.; Brodelius, P. |
title |
Elicitor-induced tyrosine decarboxylase in berberine-synthesizing suspension cultures of Thalictrum rugosum |
journal |
Eur J Biochem |
Activity |
4.1.1.28 |
ui |
88111654 |
year |
(1988) |
volume |
170 |
number |
3 |
pages |
661-6. |
| |
keywords |
Berberine/*biosynthesis |
abstract |
Tyrosine decarboxylase (EC 4.1.1.25) was induced in suspension cultures of Thalictrum rugosum by treatment with a yeast glucan elicitor. Maximum induction was observed at a carbohydrate concentration of 0.4 mg/g fresh weight of cells and maximum enzyme activity was reached 20 h after addition of elicitor. The enzyme was inducible in late exponential and early stationary growth phases. A good correlation between induced tyrosine decarboxylase activity and berberine biosynthesis has been established. It is suggested that tyrosine decarboxylase may be a key enzyme between primary and secondary metabolisms in the biosynthesis of norlaudanosoline-derived alkaloids. |
last changed |
2004/05/24 18:43 |
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