|
type |
Journal Article |
authors |
Gorin, F. A.; Mullinax, R. L.; Ignacio, P. C.; Neve, R. L.; Kurnit, D. M. |
title |
McArdle's & Hers' diseases: glycogen phosphorylase transcriptional expression in human tissues |
journal |
J Neurogenet |
Activity |
2.4.1.1 |
ui |
88155137 |
year |
(1987) |
volume |
4 |
number |
6 |
pages |
293-308. |
| |
keywords |
Amino Acid Sequence |
abstract |
We have cloned the cDNA encoding human liver glycogen phosphorylase (glycogenosis type VI) from a fetal brain cDNA library. Liver(L) and muscle(M) phosphorylase cDNA probes were used to determine the relative abundance of mRNA encoding the L- and M-isozymes of phosphorylase in human fetal and adult tissues. The transcript encoding the M-isozyme is 3.4 kb; the L-isozyme transcript is 3.3 kb. Transcriptional expression of the L-isozyme in human and primate tissues was found to differ from the isozyme's reported tissue specificity in non-primate mammals. Furthermore, using degenerate oligonucleotide probes to two different coding regions of M-phosphorylase, a novel 4.1-kb transcript was demonstrated to be present in human fetal and adult brain. |
last changed |
2002/11/12 16:17 |
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