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B6db references: 88326241

type Journal Article
authors Nishigaki, I.; Ichinose, H.; Tamai, K.; Nagatsu, T.
title Purification of aromatic L-amino acid decarboxylase from bovine brain with a monoclonal antibody
journal Biochem J
ui 88326241
year (1988)
volume 252
number 2
pages 331-5.
keywords Adrenal Medulla/enzymology
abstract Aromatic L-amino acid decarboxylase was purified from bovine brain for the first time by affinity chromatography using a monoclonal antibody to the enzyme, and it was compared with the decarboxylase purified from bovine adrenal medulla by the same procedure. The monoclonal antibody was produced from a hybridoma established for the enzyme highly purified from bovine adrenal medulla. The Mr values of brain and adrenal-medulla enzyme were both estimated to be approx. 100,000 by gel- permeation chromatography. SDS/polyacrylamide-gel electrophoresis revealed a single band with an apparent Mr of 50,000. Western immunoblot analysis showed that the antibody recognized each enzyme. With regard to substrate specificity, pH-dependence and effect of pyridoxal 5'-phosphate as a cofactor, both enzymes were similar.
last changed 2002/11/12 16:17

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