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B6db references: 8867384

type Journal Article
authors Muller, R.; Kuttler, E.; Lanz, C.; Drewke, C.; Schmidt, K.
title Isolation of a gene encoding cysteine synthase from Flavobacterium K3-15
journal FEMS Microbiol Lett
Activity 2.5.1.47
Family 2.5.1.47
sel selected
ui 8867384
year (1996)
volume 136
number 3
pages 305-8
 
keywords Amino Acid Sequence
abstract The cysteine synthase gene (cysK) from Flavobacterium K3-15 was cloned and sequenced. The gene exhibits 30-50% identity to known cysteine synthases on both the DNA and the amino acid levels. The pyridoxal phosphate binding site of the enzyme is part of a conserved motif comprising seven amino acids (SIKDRIA). The lys31 residue of the flavobacterial enzyme is conserved in all known cysteine synthases. The cysK gene from Flavobacterium K3-15 was heterologously expressed and the gene product identified by immunoblotting and determination of the enzyme activity.
last changed 2009/02/02 10:38

B6db references