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B6db references: 89250316

type Journal Article
authors Yam, C. F.; Tunnicliff, G.; Ngo, T. T.; Barbeau, A.
title A sensitive radiometric assay for cysteic acid decarboxylase activity in crude enzyme preparations of rat liver and brain
journal Comp Biochem Physiol B
Activity 4.1.1.15
ui 89250316
year (1977)
volume 58
number 1
pages 115-7
 
keywords Animal
abstract 1. A method is described for the synthesis of L-[U-14C]cysteic acid from L-[U-14C] cysteine hydrochloride and for its subsequent utilisation as a substrate for cysteic acid decarboxylase activity in liver and brain. 2. The enzyme determination relies on the entrapment of radio-labelled carbon dioxide in Hyamine hydroxide. 3. The assay is sensitive, reliable and convenient and is particularly suitable for measuring the activity of the decarboxylase in crude enzyme preparations.
last changed 2002/11/12 16:17

B6db references