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B6db references: 89374545

type Journal Article
authors Grimm, B.; Bull, A.; Welinder, K. G.; Gough, S. P.; Kannangara, C. G.
title Purification and partial amino acid sequence of the glutamate 1- semialdehyde aminotransferase of barley and synechococcus
journal Carlsberg Res Commun
ui 89374545
year (1989)
volume 54
number 2
pages 67-79
keywords Amino Acid Sequence
abstract Glutamate-1-semialdehyde aminotransferase (E.C. was purified from barley and the cyanobacteria Synechococcus PCC 6301. The purification procedure involved serial affinity chromatography and preparative polyacrylamide gel electrophoresis under non-denaturing conditions. The aminotransferase of these two organisms showed different mobilities in non-denaturing gels. In SDS-PAGE the enzyme from both organisms migrated as a single protein with an apparent molecular weight of 46.000 Da. An antibody against the barley enzyme cross-reacted with the cyanobacterial aminotransferase. This antibody also recognized a 17 kDa peptide cleaved from the barley protein with cyanogen bromide. Amino acid sequences of the NH2-termini revealed significant homology between the eucaryotic and cyanobacterial enzyme.
last changed 2008/05/21 16:26

B6db references