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B6db references: 90005995

type Journal Article
authors Kobayashi, T.; Nishii, M.; Takagi, Y.; Titani, K.; Matsuzawa, T.
title Molecular cloning and nucleotide sequence analysis of mRNA for human kidney ornithine aminotransferase. An examination of ornithine aminotransferase isozymes between liver and kidney
journal FEBS Lett
ui 90005995
year (1989)
volume 255
number 2
pages 300-4.
keywords Amino Acid Sequence
abstract The cDNA encoding ornithine aminotransferase (EC; OAT) was isolated from a human kidney cDNA library. The isolated cDNA contained the entire protein coding region and partial 3'- and 5'-untranslated regions. The nucleotide sequences of human kidney OAT cDNA were absolutely homologous with those of human liver OAT cDNA, and human kidney and liver OAT fused completely against the antibody to human kidney OAT in an Ouchterlony double diffusion test. These findings settled the controversy as to which characteristics of liver and kidney OAT isozymes are different. An N-terminal sequence analysis of purified mature human kidney OAT clarified that the leader peptide was cleaved between Gln-35 and Gly-36.
last changed 2002/11/04 17:41

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