type	Journal Article
authors	Awata, S.; Nakayama, K.; Suzuki, I.; Kodama, H.
title	Effect of cysteine on the inactivation of cystathionine gamma-lyase by D,L-propargylglycine
journal	Acta Med Okayama
Activity	4.4.1.1
Family	4.4.1.1
ui	90163978
year	(1989)
volume	43
number	6
pages	329-35.
keywords	Amino Acids/pharmacology
abstract	In vivo inactivation of cystathionine gamma-lyase by D,L- propargylglycine, a suicide inhibitor, was found to be less profound in rat kidney than in the liver. We investigated the cause of this difference using rat tissues. We fractionated kidney extract to characterize the substance which protected enzyme, and found that cysteine exhibits protecting action. Addition of 0.3 mM L-cysteine to the incubation mixture containing dialyzed kidney supernatant and 0.5 mM D,L-propargylglycine resulted in the protection of cystathionine gamma-lyase from the inactivation by the inhibitor. The content of cysteine in the kidney was six-fold higher than that in the liver. Thus, we have concluded that one of the reasons why the in vivo inactivation of cystathionine gamma-lyase in rat kidney was less than that in the liver is the presence of a higher concentration of cysteine in the kidney. S-Carboxymethylcysteine, a cysteine derivative, exhibited a similar, but weaker, protective effect.
last changed	2008/04/02 19:23