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B6db references: 90163978

type Journal Article
authors Awata, S.; Nakayama, K.; Suzuki, I.; Kodama, H.
title Effect of cysteine on the inactivation of cystathionine gamma-lyase by D,L-propargylglycine
journal Acta Med Okayama
ui 90163978
year (1989)
volume 43
number 6
pages 329-35.
keywords Amino Acids/pharmacology
abstract In vivo inactivation of cystathionine gamma-lyase by D,L- propargylglycine, a suicide inhibitor, was found to be less profound in rat kidney than in the liver. We investigated the cause of this difference using rat tissues. We fractionated kidney extract to characterize the substance which protected enzyme, and found that cysteine exhibits protecting action. Addition of 0.3 mM L-cysteine to the incubation mixture containing dialyzed kidney supernatant and 0.5 mM D,L-propargylglycine resulted in the protection of cystathionine gamma-lyase from the inactivation by the inhibitor. The content of cysteine in the kidney was six-fold higher than that in the liver. Thus, we have concluded that one of the reasons why the in vivo inactivation of cystathionine gamma-lyase in rat kidney was less than that in the liver is the presence of a higher concentration of cysteine in the kidney. S-Carboxymethylcysteine, a cysteine derivative, exhibited a similar, but weaker, protective effect.
last changed 2008/04/02 19:23

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