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B6db references: 90347447

type Journal Article
authors Sumi, C.; Ichinose, H.; Nagatsu, T.
title Characterization of recombinant human aromatic L-amino acid decarboxylase expressed in COS cells
journal J Neurochem
ui 90347447
year (1990)
volume 55
number 3
pages 1075-8.
keywords 5-Hydroxytryptophan/metabolism
abstract The expression vector containing the full-length cDNA of human aromatic L-amino acid decarboxylase (EC was transfected in COS cells by a modified calcium phosphate coprecipitation method. The cells transfected with plasmids that had a true direction of the cDNA gave a major immunoreactive band at 50 kDa. This expressed enzyme catalyzed the decarboxylation of L-3,4-dihydroxyphenylalanine (L-DOPA), L-5- hydroxytryptophan (L-5-HTP) and L-threo-3,4-dihydroxyphenylserine. The optimal pH of the enzyme activity with L-DOPA as a substrate was 6.5, whereas the enzyme had a broad pH optimum when L-5-HTP was used as a substrate. Addition of pyridoxal phosphate to the incubation mixture greatly enhanced the activity for both L-DOPA and L-5-HTP.
last changed 2002/11/12 16:17

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