Activities | Families | Sequences | Fold types | References | Help
B6db references: 91378957

type Journal Article
authors Halmekyto, M.; Alhonen, L.; Wahlfors, J.; Sinervirta, R.; Eloranta, T.; Janne, J.
title Characterization of a transgenic mouse line over-expressing the human ornithine decarboxylase gene
journal Biochem J
ui 91378957
year (1991)
volume 278
number Pt 3
pages 895-8.
keywords Animal
abstract We have produced several transgenic mouse lines over-expressing the human ornithine decarboxylase (ODC) gene. We have now characterized one of the transgenic lines as regards the tissue accumulation of the polyamines and the activities of their metabolizing enzymes. Among the tissues analysed, the polyamine pattern was most strikingly changed in testis and brain of the transgenic animals. ODC activity was greatly enhanced in all tissues, except kidney, of the transgenic animals. The most dramatic increase, 80-fold, was found in brain of the transgenic mice. The activities of S-adenosylmethionine decarboxylase and spermidine and spermine syntheses were likewise significantly increased in testis of the transgenic animals. The activities of the enzymes involved in the back-conversion of the polyamines, namely spermidine/spermine acetyltransferase and polyamine oxidase, were similar in the transgenic and non-transgenic animals. As analysed by reverse transcriptase/polymerase chain reaction, all the six tissues of the transgenic animals expressed human-specific ODC mRNA. Determination of the half-life of testicular ODC revealed a stabilization of the enzyme in the transgenic males.
last changed 2002/11/12 16:17

B6db references