|
type |
Journal Article |
authors |
Smith, D. M.; Thomas, N. R.; Gani, D. |
title |
A comparison of pyridoxal 5'-phosphate dependent decarboxylase and transaminase enzymes at a molecular level |
journal |
Experientia |
Activity |
2.1.2.1 |
ui |
92111685 |
year |
(1991) |
volume |
47 |
number |
11-12 |
pages |
1104-18. |
| |
keywords |
Amino Acid Sequence |
abstract |
Pyridoxal 5'-phosphate is a coenzyme for a number of enzymes which catalyse reactions at C alpha of amino acid substrates including transaminases, decarboxylases and serine hydroxymethyltransferase. Using the X-ray coordinates for a transaminase, aspartate aminotransferase, and the results of stereochemical and mechanistic studies for decarboxylases and serine hydroxymethyltransferase, an active-site structure for the decarboxylase group is constructed. The structure of the active-site is further refined through active-site pyridoxyllysine peptide sequence comparison and a 3-D catalytic mechanism for the L-alpha-amino acid decarboxylases is proposed. The chemistry of serine hydroxymethyltransferase is re-examined in the light of the proposed decarboxylase mechanism. |
last changed |
2002/11/04 17:41 |
|