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B6db references: 92111685

type Journal Article
authors Smith, D. M.; Thomas, N. R.; Gani, D.
title A comparison of pyridoxal 5'-phosphate dependent decarboxylase and transaminase enzymes at a molecular level
journal Experientia
ui 92111685
year (1991)
volume 47
number 11-12
pages 1104-18.
keywords Amino Acid Sequence
abstract Pyridoxal 5'-phosphate is a coenzyme for a number of enzymes which catalyse reactions at C alpha of amino acid substrates including transaminases, decarboxylases and serine hydroxymethyltransferase. Using the X-ray coordinates for a transaminase, aspartate aminotransferase, and the results of stereochemical and mechanistic studies for decarboxylases and serine hydroxymethyltransferase, an active-site structure for the decarboxylase group is constructed. The structure of the active-site is further refined through active-site pyridoxyllysine peptide sequence comparison and a 3-D catalytic mechanism for the L-alpha-amino acid decarboxylases is proposed. The chemistry of serine hydroxymethyltransferase is re-examined in the light of the proposed decarboxylase mechanism.
last changed 2002/11/04 17:41

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