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B6db references: 92160391

type Journal Article
authors Ono, B.; Naito, K.; Shirahige, Y.; Yamamoto, M.
title Regulation of cystathionine gamma-lyase in Saccharomyces cerevisiae
journal Yeast
ui 92160391
year (1991)
volume 7
number 8
pages 843-8.
keywords Cystathionine gamma-Lyase/*metabolism
abstract Regulation of the two enzymes in reverse trans-sulfuration was investigated in Saccharomyces cerevisiae. In wild-type strains, cystathionine gamma-lyase, but not cystathionine beta-synthase, was depressed nearly 15-fold if cells were starved for both inorganic and organic sulfur compounds. In a met17 strain which is defective of O- acetylserine and O-acetylhomoserine sulfhydrylase, the same enzyme was derepressed if organic sulfur compounds were limited; the repressive effect was in the order of glutathione greater than methionine greater than cysteine. The repressive effect of methionine was not observed, however, in a cys2 cys4 strain which is deficient of serine O- acetyltransferase and cystathionine beta-synthase, indicating that methionine itself is not the effector. The weak repressive effect of cysteine was attributed to inefficient uptake of this amino acid. Our observations indicate that cystathionine gamma-lyase is the target of regulation in reverse trans-sulfuration and that cysteine is very likely to be the effector of this regulation.
last changed 2008/04/02 19:23

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