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B6db references: 92187534

type Journal Article
authors Mawal, M. R.; Mukhopadhyay, A.; Deshmukh, D. R.
title Purification and properties of alpha-aminoadipate aminotransferase from rat liver and kidney mitochondria
journal Prep Biochem
ui 92187534
year (1991)
volume 21
number 2-3
pages 151-62
keywords 2-Aminoadipic Acid/*metabolism
abstract Recently we reported an affinity chromatography method to purify alpha- aminoadipate aminotransferase (AadAT) activity from rat kidney supernatant fraction. Using the same affinity column, we purified AadAT activities from rat kidney and liver mitochondria. The physical and kinetic properties such as pH optima, Km for substrates, molecular weight, subunit structure, isoelectric pH, electrophoretic mobility and inhibition by dicarboxylic acids of mitochondrial AadAT were similar to those of the AadAT from rat kidney supernatant fraction. These results indicate that AadAT from different subcellular fractions is structurally and immunologically identical.
last changed 2002/11/12 16:17

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