|
type |
Journal Article |
authors |
Kaneko, M.; Kontani, Y.; Kikugawa, M.; Tamaki, N. |
title |
Inhibition of D-3-aminoisobutyrate-pyruvate aminotransferase by 5- fluorouracil and alpha-fluoro-beta-alanine |
journal |
Biochim Biophys Acta |
Activity |
2.6.1.40 |
ui |
92338222 |
year |
(1992) |
volume |
1122 |
number |
1 |
pages |
45-9. |
| |
keywords |
Alanine Transaminase/antagonists & inhibitors |
abstract |
Among pyrimidine derivatives, we found that 5-fluorouracil potently inhibited purified rat liver D-3-aminoisobutyrate-pyruvate aminotransferase, whereas 5-fluorouridine did so to a much lesser extent. 5-Fluorouracil acted as a competitive inhibitor against beta- alanine with a Ki of 56 microM, and was uncompetitive against pyruvic acid, with a Ki of 73 microM. alpha-Fluoro-beta-alanine, a metabolite of 5-fluorouracil, was also a competitive inhibitor with respect to beta-alanine with a Ki of 8.0 mM. 5-Fluorouracil acted also as a competitive inhibitor of 4-aminobutyrate aminotransferase with respect to beta-alanine with a Ki value of 1.9 mM, and was uncompetitive against 2-oxoglutaric acid, with a Ki of 1.8 mM. |
last changed |
2002/11/12 16:17 |
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