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B6db references: 92378581

type Journal Article
authors Kawasaki, Y.; Hayashi, H.; Hatakeyama, K.; Kagamiyama, H.
title Evaluation of the holoenzyme content of aromatic L-amino acid decarboxylase in brain and liver tissues
journal Biochem Biophys Res Commun
ui 92378581
year (1992)
volume 186
number 3
pages 1242-8.
keywords Animal
abstract We have re-evaluated the content of the holo-form of aromatic L-amino acid decarboxylase in rat tissues. Aromatic L-amino acid decarboxylase was found to consume pyridoxal 5'-phosphate while it underwent decarboxylation-dependent transamination as a side reaction. We observed that the total dopamine formation was proportional to the amount of holoenzyme. Dopamine formation in a tissue extract, which was preincubated with pyridoxal 5'-phosphate, was compared with the same tissue sample but which was prepared without preincubation. Percentages of holo-form of aromatic L-amino acid decarboxylase obtained from such comparison were 78% for brain and 94% for liver tissues. These values were significantly higher than those reported earlier in which the decarboxylation-dependent transamination of the decarboxylase had been overlooked.
last changed 2002/11/12 16:17

B6db references