|
type |
Journal Article |
authors |
Kawasaki, Y.; Hayashi, H.; Hatakeyama, K.; Kagamiyama, H. |
title |
Evaluation of the holoenzyme content of aromatic L-amino acid decarboxylase in brain and liver tissues |
journal |
Biochem Biophys Res Commun |
Activity |
4.1.1.28 |
ui |
92378581 |
year |
(1992) |
volume |
186 |
number |
3 |
pages |
1242-8. |
| |
keywords |
Animal |
abstract |
We have re-evaluated the content of the holo-form of aromatic L-amino acid decarboxylase in rat tissues. Aromatic L-amino acid decarboxylase was found to consume pyridoxal 5'-phosphate while it underwent decarboxylation-dependent transamination as a side reaction. We observed that the total dopamine formation was proportional to the amount of holoenzyme. Dopamine formation in a tissue extract, which was preincubated with pyridoxal 5'-phosphate, was compared with the same tissue sample but which was prepared without preincubation. Percentages of holo-form of aromatic L-amino acid decarboxylase obtained from such comparison were 78% for brain and 94% for liver tissues. These values were significantly higher than those reported earlier in which the decarboxylation-dependent transamination of the decarboxylase had been overlooked. |
last changed |
2002/11/12 16:17 |
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