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B6db references: 93016579

type Journal Article
authors Ide, H.; Hamaguchi, K.; Kobata, S.; Murakami, A.; Kimura, Y.; Makino, K.; Kamada, M.; Miyamoto, S.; Nagaya, T.; Kamogawa, K.; et al.
title Purification of serine hydroxymethyltransferase from Bacillus stearothermophilus with ion-exchange high-performance liquid chromatography
journal J Chromatogr
ui 93016579
year (1992)
volume 596
number 2
pages 203-9.
keywords Ammonium Sulfate
abstract The gene of serine hydroxymethyltransferase (SHMT) of a thermophilic bacterium Bacillus stearothermophilus was expressed in Escherichia coli, and SHMT was successfully purified from the crude extract of E. coli in two steps while maintaining the enzymatic activity. The purification steps involved ammonium sulphate precipitation followed by high-performance liquid chromatographic separation using the anion- exchange column Fractogel EMD DEAE-650(S). In addition to the DEAE column, three other types of anion- and cation-exchange columns were also studied for their ability to separate SHMT, and the performance of the four columns were compared.
last changed 2002/11/04 17:41

B6db references